For MetaXpress High Content Image Acquisition and Analysis Software
The Custom Module Editor in version 5 of MetaXpress® Software is a flexible, interactive environment where users can create and test a template for image analysis. Galleries of processing options, morphology filters, application modules, and segmentation methods are available for users to construct a sequence of analysis steps. The new Click-to-Find Tool enables easy object identification and auto-populates step card parameters. Once created, custom modules can be easily shared and re-used to analyze other image sets.
Custom modules can be rapidly written to measure general and specific toxicity parameters. In addition to monitoring cell viability, apoptosis, autophagy and mitochondria membrane potential, a custom module allows you to simultaneously analyze tissue-specific toxic effects. The user decides which responses to detect and also which measurements to record out.
The Custom Module Editor enables more analyses, such as identifying objects within objects, creating morphometric classifiers for shape analysis, and analyzing both color and transmitted light images. With custom modules, researchers can simplify segmentation steps and report only on the data selected, saving unnecessary analysis time. Analysis time can be further accelerated by taking advantage of parallel processing using MetaXpress® PowerCore™ Software.
Creating a custom module
Shown is a sample of a custom module to find granules in nuclei using the Custom Module Editor.
- Select raw image in the segment tab.
- Identify nuclei using the new Click-to-Find tool. Simply click on objects of interest and measurement parameters are updated automatically.
- Find spots with granule detection.
- Measure only granules within the nuclei mask.
Example measurements from a multi-parameter hepatotoxicity assessment:
- Total number of nuclei
- Number of apoptotic nuclei
- Size of nuclei
- Number of live cells (viability)
- Number of mitochondria/cell (counting granules in cytoplasm)
- Total amount of phospholipids/cell