Cellular Imaging

Cellular Imaging

Whether the demand is for a high-content and high-throughput or a simple-to-use system, ImageXpress Micro Confocal, ImageXpress Micro 4, ImageXpress Nano, or ImageXpress Pico can accommodate acquisition and analysis that suits the needs of a screening or research lab.

Cellular imaging is a fundamental technique in life sciences research that combines qualitative information about cellular appearance with quantitative data about cellular characteristics. Researchers have several options in imaging methods, from phase-contrast microscopy that shows intact cells to fluorescent imaging of single molecules or organelles. Brightfield microscopy is useful for visualizing whole cells rapidly, whereas confocal microscopy is preferred for high-quality images and 3D reconstruction of cells. Researchers interested in molecular or cellular dynamics can perform 4D live-cell imaging that combines confocal with time-lapse microscopy. Automated microscopy employs software and hardware features which ensure robust focus in every field-of-view whether on a slide or a multi-well microplate and, combined with sophisticated image analysis software, generate repeatable and subjective quantitative data. Cellular images are not only captured and stored for visualization but measurements such as cell count, size or shape, translocation, and expression level can be routinely quantitated.

  • Organ-on-a-Chip Assays

    Emulating organ physiology by co-culturing cells in a supportive 3D matrix and using microfluidic channels to perfuse nutrients or compounds over the resulting cellular structures, is rapidly gaining popularity as a biologically relevant screening model for new drugs or toxicity. The ImageXpress system provides a turnkey solution for both 2D or 3D applications and can accommodate many different types of slide and plate formats.

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    Cell Migration Assay

    The movement or migration of cells is often measured in vitro to elucidate the mechanisms of various physiological activities such as wound healing or cancer cell metastasis. Cell migration assays may be conducted in a controlled environment using live cell timelapse imaging.

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  • Cardiomyocytes

    Stem cells differentiated into cardiomyocytes are used to screen early for potential toxicological effects of drugs, thus helping to avoid investment in development of drugs which will fail in clinical trials due to cardiac toxicity.

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    Neurite Outgrowth / Neurite Tracing

    Neurons create connections via extensions of their cellular body called processes. This biological phenomenon is referred to as neurite outgrowth. Understanding the signaling mechanisms driving neurite outgrowth provides valuable insight into neurotoxic responses, compound screening, and for interpreting factors influencing neural regeneration. Using the ImageXpress Micro system in combination with MetaXpress Image Analysis Software automated neurite outgrowth imaging and analysis is possible for slide or microplate-based cellular assays.

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  • 3D Biology

    The development and integration of three-dimensional (3D) assay models are becoming popular to drive translational biology. Our ImageXpress® Micro Confocal High-Content Imaging System allows superior visualization of thick samples by minimizing background fluorescence and increasing sharpness, resulting in accurate image segmentation. Increase the biological relevance of your screening assays with a seamless integration between acquisition and analysis of cells in a 3D space to yield volume, intensity, and distance measurements.

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    Spheroid Assays

    Three-dimensional (3D) cells cultured into spheroids provide a more complete and physiologically predictive model especially for cancer research. Acquiring measurements from the larger structure involves acquiring images from different depths within the body of the spheroid and analyzing them in 3D or collapsing into a single 2D stack before analysis. The ImageXpress Micro system and MetaXpress software are designed to make acquisition and analysis of 3D spheroids fast and accurate.

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  • Stem Cell Research

    Pluripotent stem cells can be used for studies in developmental biology or differentiated as a source for organ-specific cells and used for live or fixed cell-based assays on slides or in multi-well plates. The ImageXpress Nano system paired with CellReporterXpress software for analysis has utility in all parts of the stem cells researcher’s workflow from tracking differentiation to quality control to measuring functionality of specific cell types.

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    Data Analysis [AcuityXpress]

    With the growing use of high content screening in drug discovery, informatics has come to the forefront as a critical technology to effectively utilize the massive volumes of high content data and images being generated. AcuityXpress™ Software was specifically designed to address the needs of high-content data analysis by providing multi-parametric cellular or summary results multiple microplates. AcuityXpress Software shares the MDCStore™ Database with MetaXpress® Software for seamless integration of images and analyzed data.

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  • 3D Cell Imaging and Analysis

    Three-dimensional (3D) cell models are physiologically relevant and more closely represent tissue microenvironments, cell-to-cell interactions, and biological processes that occur in vivo. Now you can generate more predictive data by incorporating technologies like the ImageXpress system with the integrated 3D Analysis Module in MetaXpress® software. This single interface will enable you to meet 3D acquisition and analysis challenges without compromise to throughput or data quality, giving you confidence in your discoveries.

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    Toxicity Screening

    Screening for off-target or toxic effects is very important during the development of new drugs and for the extension of the therapeutic potential of existing molecules. ImageXpress systems are fully integrated hardware and software platforms for automated acquisition and analysis of images for high-throughput cell-based cytotoxicity testing. Configured with optional environmental control, living cell responses or kinetic reactions can be monitored in real time for several days.

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