Application Modules for MetaXpress and
MetaXpress PowerCore Software

Application modules for the MetaXpress^® and MetaXpress^® PowerCore™ software allows users to easily automate common analysis tasks. The following modules are now available:

• Angiogenesis tube formation
• Cell cycle
• Cell health
• Cell proliferation HT
• Cell scoring
• Count nuclei
• Granularity
• Live/dead
• Micronuclei
• Mitotic Index
• Monopole detection
• Multi wavelength cell scoring
• Multi wavelength translocation
• Neurite outgrowth
• Nuclear translocation HT
• Transfluor®
• Transfluor HT
• Translocation — basic and enhanced

Application modules feature:

• Intuitive dialog boxes
• Adaptive Background Correction - Improved segmentation through adaptation to local content
• Assay can be run across entire data set
• Field and cell-by-cell data logging
• Compatibility with MetaXpress PowerCore high throughput image analysis
• Customization through MetaMorph^® software journals (not compatible with MetaXpress PowerCore software)

• Endothelial tube formation: Angiogenesis tube formation
• Promotion or inhibition of blood vessel formation: Angiogenesis tube formation
• Cell viability: Live/dead, Cell cycle, Multi Wavelength Cell Scoring
• Cell cycle: Mitotic Index, Cell cycle
• Membrane potential: Cell health
• Cytotoxicity and apoptosis: Live/dead
• Cell proliferation: Count nuclei, Cell Proliferation HT, Live/dead
• Cell migration: Count nuclei, Cell Proliferation HT
• Cell counting: Count nuclei, Cell Proliferation HT
• Determination of cell health and proliferation in populations of mono-, bi- and multi-nucleated cells: Micronuclei
• Micronuclei and genotoxicity analysis: Micronuclei
• Kinase activation: Cell scoring
• Fatty acid uptake: Cell scoring, Multi Wavelength Cell Scoring
• Mitosis: Mitotic Index
• Adipogenesis: Cell scoring, Multi Wavelength Cell Scoring
• Examining transfection efficiencies: Cell scoring, Multi Wavelength Cell Scoring
• Studying intracellular structures: Transfluor, Transfluor HT, Granularity
• Receptor internalization: Transfluor, Transfluor HT, Granularity
• Punctate staining: Transfluor, Transfluor HT, Granularity
• Clustering target molecules: Transfluor, Transfluor HT, Granularity
• Process extension: Neurite outgrowth
• Neurodegenerative or neuroregenerative disease research: Neurite outgrowth
• Cell differentiation (Stem cell research): Neurite outgrowth
• Protein movement: Translocation, Nuclear Translocation HT, Multi Wavelength Translocation
• Cell pathway analysis: Translocation, Nuclear Translocation HT
• Protein phosphorylation: Cell scoring, Multi Wavelength Cell Scoring
• Mitochondrial localization: Translocation
• Detect monopolar spindles: Monopole detection

• Classify Micro- and Multi-nucleated cells
• Additional markers for analysis of cytotoxicity
• More than 100 outputs
• Fastest "acquisition through analysis" workflow with on-the-fly cell counting and MetaXpress^® PowerCore™ high-throughput image analysis

• Better quantitation by creating a single in-focus composite image from multiple Z-series images
• Multi-parameter analysis measurements include tube length, number of branch points, number of nodes and more

Figure 1. Inhibition of Human Mammary Epithelial Cells (HMEC-1) tube formation by suramin. BD BioCoat™ Angiogenesis System. Data courtesy of BD Biosciences.

• Quantitation of cell cycle stages
• Optional apoptosis and mitotis-specific stains

Figure 2. Cell cycle classification can be performed with a single or multi wavelength assay. Left: CHO-K1 cells stained with Hoechst 33342 were imaged with a 20x objective on the Discovery-1 system using MetaXpress software. Right: The Cell Cycle module identifies cell cycle phases: G0/G1 (dark blue), S (light blue), G2 (green), Early M (orange) and Late M (red).

• Analysis of up to three fluorescent probes for cell-based assays of apoptosis and necrosis
• Multi-parameter analysis measurements include counts and percentages of viable, early and late apoptotic and necrotic cells and more

Figure 3. Adherent Chinese Hamster Ovary (CHO-K1) cells were incubated with various concentrations of staurosporine for 6-12 hours. Top left: control, top right: 0.1 µM staurosporine, bottom left: 3 µM staurosporine, bottom right: measured (Green: viable, blue: early apoptotic, purple: late apoptotic, red: necrotic).

• Identification of two sub-population of cells
• Ideal for counting and logging measurements of cells in two-wavelength experiments

Figure 4. The module can be used as a generic segmentation tool to identify two different stains (top) or to obtain cell details (bottom).

• Automatically counts nuclei and captures intensity measurements
• Accurate segmentation of touching cells

Figure 5. Left: Image has uneven illumination and touching cells. Right: Count Nuclei identifies touching cells as separate objects (as indicated by the red arrow). Adaptive Background Correction™ compensates for the variable background intensity – even when background intensity in one area of the image is greater than nuclei intensity in another area.

• Quantitation of live and dead cells
• Multi-parameter analysis measurements include quantitation and percentages of live and dead cells, per-wavelength measurements and more

Figure 6. Adherent Chinese Hamster Ovary (CHO-K1) cells were incubated with varying concentrations of DMSO for 6-12 hours prior to staining. Cells were labeled with H33342 and PI diluted in PBS for 30-60 minutes @RT before image acquisition. Top left: control, top right: 20% DMSO, bottom: measured (red: dead, green: live).

• Quantitation and percentage of mitotic and interphase cells
• Measurements include count, percentage and wavelength-specific intensities of mitotic and non-mitotic nuclei, and more

Figure 7. Left: CHO-K1 cells treated with Nocadazole for 18 hours before staining with anti-phospho-Histone H3 (Ser28) and acquired by Discovery-1. Right: mitotic (green) and interphase (red) cells.

• Quantitation of mitotic cells with monopolar or bipolar spindles
• Measurements include count and percentage of monopoles, bipoles and interphase cells, DNA structures area, cell classification and more

Figure 8. 3T3-L1 mouse fibroblast cells treated with monastrol and stained with mouse anti-beta tubulin primary antibody detected with a FITC conjugated goat anti-mouse secondary antibody. Nuclei are stained with Hoeschst 33342. Left: monastrol, right: segmented image shows interphase cells (red), bipolar spindles (blue) and monopole (green).

• Multi-parameter analysis measurements include total neurite outgrowth, total branches and cell bodies, straightness and more
• Works with or without nuclear stain

Figure 9. Top: DF cells. Bottom: DF cells skeletonized. Data courtesy of Rinat Neuroscience Corporation.

• Designed to facilitate the analysis of receptor internalization specifically for the Transfluor assay
• Intensity measurements and quantitation of pits, vesicles and nuclei
• Choice of six granularity indices

Figure 10. Human osteosarcoma cells (U2OS) incubated overnight for 40-45 minutes (vesicle formers) or 30 minutes (pit formers). Varying concentrations of isoproterenol were added to one set of wells. Top: acquisition, bottom: Pits (white), vesicles (red) and nuclei (green) can be simultaneously segmented. Grains are accurately identified even in cells with high background (red arrow).

• Quantitation of correlation between probes and compartments
• Two modules to fit your needs: the Translocation module, ideal for images that need a minimum amount of adjustments, and the Translocation-Enhanced module, for additional flexibility
• Measurements include compartments, mean compartment area, correlation coefficient, intensity, number and percentage of positives, and more

Figure 11. Mitochondrial translocation. HeLa cells treated with Staurosporine and stained with MitoTracker (red), DAPI (blue) and anti-Cytochrome C (green).

Product Name	Part Number
MetaXpress Offline Analysis Station with MDCStore Integrated Database Functionality	METAX OFFLINE
Angiogenesis Tube Formation Application Module	9500-0016
Cell Cycle Application Module	9500-0040
Cell Health Application Module	9500-0035
Cell Proliferation HT Application Module	9500-0042
Cell Scoring Application Module	9500-0037
Count Nuclei Application Module	9500-0017
Granularity Application Module	9500-0032
Live/Dead Application Module	9500-0034
Micronuclei Application Module	9500-0045
Mitotic Index Application Module	9500-0036
Monopole Detection Application Module	9500-0039
Multi Wavelength Cell Scoring Application Module	9500-0038
Multi Wavelength Translocation Application Module	9500-0044
Neurite Outgrowth Application Module	9500-0015
Nuclear Translocation HT Application Module	9500-0041
Transfluor Application Module	9500-0033
Transfluor HT Application Module	9500-0043
Translocation Application Modules	9500-0014

To place an order, call your local Molecular Devices Office or Molecular Devices Distributor.