SpectraMax M5e Multi-Mode Microplate Reader
The SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays.
The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters.
The SpectraMax M5e Micoplate Reader possesses the five modes preferred by the majority of our users providing the benefit of multiple detection modes in one platform.
- UV-Visible Absorbance (Abs)
- Fluorescence Intensity (FI)
- Time-Resolved Fluorescence (TRF) including CisBio HTRF Certification
- Fluorescence Polarization (FP)
- Glow Luminescence (Lumi)
The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates.
Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed optical pathlength cuvette. This temperature-independent normalization corrects for varying well volumes and can eliminate standard curves by allowing the calculation of concentrations directly from the absorbance with a known extinction coefficient, as exemplified with nucleic acid and protein quantitation.
For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5e Micoplate Reader optical design provides the highest level of flexibility. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. Assays can be better optimized by scanning across a range of wavelengths in increments as small as 1 nm. Up to 4 wavelength pairs can be read in one protocol for endpoint and kinetic measurements, allowing for fast setup of FRET and TR-FRET assays, including CisBio's HTRF assay.
For luminescence, the SpectraMax M5e Microplate Reader utilizes a dedicated luminescence photomultiplier tube (PMT), providing the user the maximum signal and lowest background possible for glow luminescence reporter gene assays.
The SpectraMax M5e Microplate Reader is supplied with SoftMax Pro Data Acquisition & Analysis Software, Molecular Devices' industry leading all-in-one software. Among the various ready-to-read protocols, users will find pre-written SpectraDrop™ Plate protocols enabling Micro Volume applications measurement. Additionally, the SpectraMax M5e Micoplate Reader can be seamlessly integrated with the StakMax Microplate Handling Stacker through SoftMax Pro Microplate Data Acquisition & Analysis Software.
Multiple Detection Modes. The optics in the SpectraMax M5e Micoplate Reader are designed to easily adapt to changes in assay requirements while maintaining the optimal performance for all modes including absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization.
Precision Optics and Detectors.
The SpectraMax M5e Micoplate Reader utilizes several key technologies to ensure optimal sensitivity for all modes.
- Dual monochromators allow users to target the optimal assay excitation and emission wavelengths and eliminate the need to change expensive band pass filters between experiments.
- Dual photomultiplier tubes (PMTs) provide detection flexibility to the widest variety of assays.
- Reference diodes automatically adjust to slight fluctuations in excitation intensity to reduce measurement noise.
- Absorbance applications are enhanced using top-quality UV grade fibers to provide strong light transmission in the lowest wavelengths.
Patented AutoPMT Optimization System
Unlike most fluorescence readers that may saturate with signal intensities over 3 orders of magnitude, the patented AutoPMT System of the SpectraMax M5e Microplate Reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the dynamic range of assays so that low, medium, and high signals can be captured from the same plate. This calibration against an internal standard provides an additional benefit in being able to confidently compare relative fluorescence units (RFUs) of individual samples across plates and readers.
Patented PathCheck Pathlength Measurement Technology
Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples in a temperature independent manner using the patented PathCheck® Technology. With SoftMax Pro Data Acquisition & Analysis Software, the PathCheck Technology automatically normalizes the well absorbance.
Comprehensive Reader Validation
Molecular Devices microplate readers have the most complete level of product validation and compliance of any system on the market. A variety of tools are available to users to meet users' diverse validation needs. Molecular Devices provides the complete solution covering the instrument and software.
- SpectraTest® ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes
- IQ/OQ/PQ documentation
- SoftMax Pro Data Acquisition & Analysis Software validation package
- Software tools for FDA 21 CFR Part 11 compliance
Automating with Ease
The SpectraMax M5e Microplate Reader has several options when a user's throughput needs demand walk-away automation.
Seamless integration with the StakMax Microplate Handling Stacker, Molecular Devices' own integrated microplate stacker designed exclusively for SpectraMax Microplate Readers and AquaMax® Microplate Washers.
For more complex automation needs, SpectraMax M5e Microplate Reader has an automation-compatible reader design for integration into automated platforms. Molecular Devices' microplate readers have been integrated into all major automation platforms.
SpectraMax® M5 Reader has the right stuff.
SpectraDrop™ Micro-Volume Microplates
Low volume solution for benchtop microplate readers.
- M-Series Product Data Sheet
- BioResearch Products Data Sheet
- SoftMax Pro Software Data Sheet
- SpectraTest Validation Data Sheet
- Application Notes