Microplate Readers

SpectraMax M4 Multi-Mode Microplate Reader

Provides four modes of detection in a multi-mode system with an upgrade option to add FP and HTRF modes at a later time. Your applications, your modes, your choice.

The SpectraMax® M4 Multi-Mode Microplate Reader dual monochromator optics allow the widest range of applications without the need to change filters and delivers single-mode performance in one multi-mode platform with the following detection modes:

  • UV-Visible Absorbance (Abs)
  • Fluorescence Intensity (FI)
  • Glow Luminescence (Lumi)
  • Time-Resolved Fluorescence (TRF)

The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates.

Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M4 Microplate Reader transforms each well in a microplate to a fixed optical pathlength cuvette and can eliminate standard curves by allowing the calculation of concentrations directly from the absorbance with a known extinction coefficient, as exemplified with nucleic acid and protein quantitation.

For fluorescence intensity and time-resolved fluorescence assays, users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. Assays can be better optimized by scanning across a range of wavelengths in increments as small as 1 nm. Up to 4 wavelength pairs can be read in one protocol for endpoint and kinetic measurements, allowing for fast setup of FRET and TR-FRET assays.

For luminescence, the SpectraMax M4 Microplate Reader utilizes a dedicated luminescence photomultiplier tube (PMT), providing the user the maximum signal and lowest background possible for glow luminescence reporter gene assays.

If the needs of the researcher change, the SpectraMax M4 Microplate Reader can be upgraded with more detection options including fluorescence polarization and HTRF certified detection making it equivalent to the SpectraMax M5 Microplate Reader or the SpectraMax M5e Microplate Reader.

The SpectraMax M4 Microplate Reader is supplied with SoftMax® Pro Data Acquisition & Analysis Software, Molecular Devices' industry leading all-in-one software. Among the various ready-to-read protocols, users will find pre-written SpectraDrop™ Plate protocols enabling Micro Volume applications measurement. Additionally, the SpectraMax M4 Microplate Reader can be seamlessly integrated with the StakMax® Microplate Handling Stacker through SoftMax Pro Software.


Multiple Detection Modes:

Precision Optics and Detectors: The SpectraMax M4 Microplate Reader utilizes several key technologies to ensure optimal sensitivity for all modes.

  • Dual monochromators allow users to target the optimal assay excitation and emission wavelengths and eliminate the need to change expensive band pass filters between experiments.
  • Dual photomultiplier tubes (PMTs) provide detection flexibility to the widest variety of assays.
  • Reference diodes automatically adjust to slight fluctuations in excitation intensity to reduce measurement noise.
  • Absorbance applications are enhanced using top-quality UV grade fibers to provide strong light transmission in the lowest wavelengths.

Patented AutoPMT Optimization System: Unlike most fluorescence readers that may saturate with signal intensities over 3 orders of magnitude, the patented AutoPMT Optimization System of the SpectraMax M4 Microplate Reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the dynamic range of assays so that  low, medium, and high signals can be captured from the same plate. This calibration against an internal standard provides an additional benefit in being able to confidently compare relative fluorescence units (RFUs) of individual samples across plates and readers.

Patented PathCheck® Pathlength Measurement Technology: Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples in a temperature independent manner using the patented PathCheck® Technology. With SoftMax Pro software, the PathCheck Technology automatically normalizes the well absorbance.

Comprehensive Reader Validation: Molecular Devices microplate readers have the most complete level of product validation and compliance of any system on the market. A variety of tools are available to users to meet users' diverse validation needs. Molecular Devices provides the complete solution covering the instrument and software.

Automating with Ease: The SpectraMax M4 Microplate Reader has several options when user throughput needs demand walk-away automation. Seamless integration with the StakMax Stacker, Molecular Devices' own integrated microplate stacker designed exclusively for SpectraMax Microplate Readers and AquaMax® Microplate Washers.

For more complex automation needs, SpectraMax M4 Microplate Reader has an automation-compatible reader design for integration into automated platforms.  Molecular Devices' microplate readers have been integrated into all major automation platforms.

The optics in the SpectraMax M4 Microplate Reader are designed to easily adapt to changes in assay requirements while maintaining the optimal performance for all modes including absorbance, fluorescence intensity, luminescence, and time-resolved fluorescence.
Contact Us

Turn ideas into discoveries.

Request Quote

What's New?
ScanLater Western Blot Detection System

NEW ScanLater Western Blot Detection System - Enables western blot and ELISA detection on your microplate reader. Learn more.

The Right Stuff
SpectraMax M5 on International Space Station

SpectraMax® M5 Reader has the right stuff.
Learn more

Low Volume Solution

SpectraDrop™ Micro-Volume Microplates

Low volume solution for benchtop microplate readers.

Download datasheet