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SpectraMax M2 & M2e Multi-Mode Microplate Reader

Premier dual-mode readers for absorbance & fluorescence intensity-based assays

The SpectraMax® M2 & M2e Multi-Mode Microplate Readers provide two detection modes in one platform and has the the added functionality of bottom read fluorescence for cell-based assays.

  • UV-Visible Absorbance (Abs)
  • Fluorescence Intensity (FI- Top Read)

The SpectraMax M2 and M2e Microplate Readers are the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates.

Using the patented, PathCheck® PathLength Measurement Technology, the SpectraMax M2 & M2e Microplate Readers transform each well in a microplate to a fixed optical pathlength cuvette, and can eliminate standard curves by allowing the calculation of concentrations directly from the absorbance with a known extinction coefficient, as exemplified with nucleic acid and protein quantitation.

For fluorescence intensity assays, the SpectraMax M2 and M2e Microplate Readers optical design provides the highest level of flexibility. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays (M2e only).  Assays can be better optimized by scanning across a range of wavelengths in increments as small as 1 nm.  Up to 4 wavelength pairs can be read in one protocol for endpoint and kinetic measurements, allowing for fast setup of FRET assays.

The SpectraMax M2 and M2e Microplate Readers are supplied with SoftMax® Pro Data Acquisition & Analysis Software, Molecular Devices' industry leading all-in-one software.  Among the various ready-to-read protocols, users will find pre-written SpectraDrop™ Plate protocols enabling Micro Volume applications measurement. Additionally, the SpectraMax M2 and M2e Microplate Readers can be seamlessly integrated with the StakMax® Microplate Handling Stacker through SoftMax Pro Software.


Dual Detection Modes: The optics in the SpectraMax M2 and M2e Microplate Readers are designed to easily adapt to changes in assay requirements while maintaining the optimal performance for absorbance and fluorescence intensity.

Precision Optics and Detectors: The SpectraMax M2 & M2e Microplate Reader utilize several key technologies to ensure optimal sensitivity for both modes.

Dual monochromators allow users to target the optimal assay excitation and emission wavelengths and eliminate the need to change expensive band pass filters between experiments.

Reference diodes automatically adjust to slight fluctuations in excitation intensity to reduce measurement noise.

Absorbance applications are enhanced using top quality UV grade fibers to provide strong light transmission in the lowest wavelengths.

Patented AutoPMT Optimization System: Unlike most fluorescence readers that may saturate with signal intensities over 3 orders of magnitude, the patented AutoPMT Optimization System of the SpectraMax M2 Microplate Reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the dynamic range of assays so that  low, medium, and high signals can be captured from the same plate.  This calibration against an internal standard provides an additional benefit in being able to confidently compare relative fluorescence units (RFUs) of individual samples across plates and readers.

Patented PathCheck® Path Length Measurement Technology: Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples in a temperature independent manner using the patented PathCheck® Technology. With SoftMax Pro Software, the PathCheck Technology automatically normalizes the well absorbance.

Comprehensive Reader Validation: Molecular Devices microplate readers have the most complete level of product validation and compliance of any system on the market.  A variety of tools are available to users to meet users' diverse validation needs. Molecular Devices provides the complete solution covering the instrument and software.

SpectraTest® ABS1 and FL1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes

Automating with Ease: The SpectraMax M2 and M2e Microplate Readers have several options when user throughput needs demand walk-away automation.  Seamless integration with the StakMax Stacker, Molecular Devices' own integrated microplate stacker designed exclusively for SpectraMax Microplate Readers and AquaMax® Microplate Washers.

For more complex automation needs, the SpectraMax M2 Microplate Reader has an automation-compatible reader design for integrated into automation platforms.  Molecular Devices' microplate readers have been integrated into all major automation platforms.

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