Cart

Comparison of Microplate Readers with Luminescence Detection

 

 

Features
Detector
Spectral range
Dynamic range
Sensitivity
Microplate format
Cross-talk
Read modes
Read times
Injectors/pipettors
Injection/pipettor volumes
Temperature control
Software
Validation & compliance tools
Robotic compatible
Detector
Photomultiplier tubes (PMT) and/or photodiode
Spectral range
300-850 nm
Dynamic range
> 6 decades
Sensitivity
3 pM for 96-wells, 6 pM for 384-wells (Optimized for ATP-Glow)
Microplate format
6 to 1536 wells*
Cross-talk
< 0.4% in white 96- and 384-well microplates
Read modes
Spectral, Endpoint, Kinetic
Read times
0:30 minutes for 96-wells, 1:15 minutes for 384-wells
Injectors/pipettors
Option available
Injection/pipettor volumes
 
Temperature control
2 degrees C above ambient to 45 degrees C
Software
SoftMax Pro Software
Validation & compliance tools
IQ/OQ Protocols, SoftMax Pro GxP Compliance Software, SpectraTest LM1 Validation Plate
Robotic compatible
Detector
2 PMT
Spectral range
300-850 nm
Dynamic range
> 6 decades
Sensitivity
1.25 pM for 96-wells, 3 pM for 384-wells, 19 pM for 1536-wells (Optimized for ATP-Glow)
Microplate format
6 to 1536 wells
Cross-talk
 
Read modes
Endpoint, Kinetic
Read times
 
Injectors/pipettors
Option available
Injection/pipettor volumes
 
Temperature control
2 degrees C above ambient to 45 degrees C
Software
SoftMax Pro Software
Validation & compliance tools
SoftMax Pro GxP Compliance Software
Robotic compatible
 
Detector
Photo-multiplier tube
Spectral range
250-850 nm
Dynamic range
> 6 decades
Sensitivity
43 pM ATP in 96 wells (optimized)
Microplate format
6-384-well microplates
Cross-talk
< 0.3% in white 96- and 384-well microplates
Read modes
Endpoint, Kinetic
Read times
2:00 minutes for 96-wells, 7:00 minutes for 384-wells
Injectors/pipettors
 
Injection/pipettor volumes
 
Temperature control
2 degrees C above ambient to 45 degrees C
Software
SoftMax Pro Software
Validation & compliance tools
IQ/OQ Protocols, FDA 21 CFR Part 11 Compliance, SpectraTest LM1 Validation Plate
Robotic compatible
 
Detector
2 PMT
Spectral range
250-850 nm
Dynamic range
> 6 decades
Sensitivity
< 2 fg/well for firefly luciferase in 96- and 384-well top read
Microplate format
6-384 (96- and 384-wells with pipetting)
Cross-talk
< 0.3% in white 96- and 384-well microplates
Read modes
Endpoint, Kinetic, Fast Kinetic
Read times
2:00 minutes for 96-wells, 7:00 minutes for 384-wells
Injectors/pipettors
8- or 16-channel pipettor
Injection/pipettor volumes
Max. volume 200uL for 8-channel and 30 uL for 16-channel
Temperature control
2 degrees C above ambient to 45 degrees C
Software
SoftMax Pro Software
Validation & compliance tools
IQ/OQ/PQ Protocols, FDA 21 CFR Part 11 Compliance
Robotic compatible
 
Detector
1, or 2 Ultra-fast Photomultiplier Tubes
Spectral range
380-630 nm
Dynamic range
> 9 decades
Sensitivity
< 20 attomol ATP per well
Microplate format
96- and 384-well microplates
Cross-talk
< 3 x 10-5 (96-well)
Read modes
Endpoint, Kinetic, Fast Kinetic, Dual-read
Read times
0.1 up to 3600 sec. in steps of 0.1 sec.
Injectors/pipettors
2 variable volume injectors
Injection/pipettor volumes
 
Temperature control
5 degrees C above ambient to 45 degrees C
Software
SoftMax Pro Software
Validation & compliance tools
Software Validation Package, IQ/OQ Documentation, Physical Validation Test Plate
Robotic compatible
 
Detector
Silicon Photodiode + PMT
Spectral range
F3: 400 - 650nm FilterMax F5: 400 - 750 nm
Dynamic range
6 decades
Sensitivity
 
Microplate format
F3: 96 - 384 well F5: 6 - 1536 well
Cross-talk
 
Read modes
Endpoint, Kinetic, Linear Scan, Area Scan
Read times
18sec/96well 37sec/384well
Injectors/pipettors
 
Injection/pipettor volumes
 
Temperature control
 
Software
SoftMax Pro Software
Validation & compliance tools
IQ/OQ Protocols, SoftMax Pro GxP Compliance Software, SpectraTest LM1 Validation Plate
Robotic compatible
 

 

* 1536 detection available via detection cartridges