SpectraMax Gemini EM Microplate Reader

Re-invent your fluorescence assays

Create custom fluorescence assays without having to purchase expensive filter sets. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets you determine the optimal excitation and emission settings for your creative new fluorescence intensity assays. If the best wavelengths are unknown, the Gemini EM reader will find them for you. Switch easily between top and bottom reading modes for solution- and cell-based assays.


  • Read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes
  • Eliminate the need for identifying, purchasing, and changing out filters for wavelength optimization with dual monochromators for excitation and emission wavelength selection between 250 and 850 nm
  • Measure fluorescence from solutions or cell-based assays with the best optical sensitivity by switching between top and bottom fluorescence reading
  • Up to 4 wavelength pairs can be read for endpoint and kinetic measurements
  • Well scanning to report a fluorescent measurement from a single point in the center of a microplate well to multiple points across a tissue culture well

Measuring Accurately and Reproducibly

  • Avoid losing high signals to detector saturation and photobleaching after repeated reads to find the correct reader settings with AutoPMT System electronics
  • Compare relative fluorescence units (RFUs) between samples, like with no other fluorometer, allowed by a unique calibration against an internal standard
  • Monitoring of temperature-sensitive reactions with consistent temperature regulation from ambient to 45°C
  • Thorough mixing of samples in microplate wells by user-programmable Automix feature
  • Gentle movement of cell or other aggregates in the microplate with a slow carriage speed option
  • Seamless automation coupling with the StakMax Stacker, Molecular Devices own integrated microplate stacker designed exclusively for the SpectraMax Reader

Comprehensive Reader Validation

Molecular Devices microplate readers have the most variety of tools available for users to meet their diverse hardware and software validation needs. Molecular Devices provides complete solutions with:

The Gemini EM Microplate Reader supports a multitude of fluorescence assay categories including:

  • ELISAs and immunoassays
  • Nucleic acid (DNA) quantitation
  • Protein quantitation
  • Reporter gene assays
  • Cell Viability, proliferation, and cytotoxicity
  • Enzyme assays
  • Transporter assays
  • Phosphotases/kinases
  • Microbial growth

SoftMax® Pro Software is the most published microplate reader control and microplate data analysis software package available to scientists. The inclusive package of ready-to-run protocols, analysis algorithms, and 21 different curve fit options provides the full solution from data acquisition to analysis to publishing. The FDA 21 CFR Part 11 compliance tools available in SoftMax Pro GxP Software for regulated laboratories deliver a complete solution to minimize validation costs, provide end-to-end chain of custody, and eliminate the need to validate additional analysis software.

General Specifications

Dimensions (in) 8.6 H x 22.8 W x 15 D
Dimensions (cm) 22 H x 58 W x 38 D
Weight 35 lbs (16 kg)
Power Source 100-240 VAC, 50/60 Hz

Performance Specifications

Wavelength Range-Excitation 250-850 nm
Wavelength Range-Emission 250-850 nm
Wavelength Selections Monochromator, tunable 1.0 nm increments
Wavelength Bandwidth (Excitation and emission) 9nm
Wavelength Accuracy < ± 2.0 nm
Top Read Sensitivity 3.0 fmol/well FITC 200 µl in 96 wells
Bottom Read Sensitivity 8.0 fmol/well FITC 200 µl in 96 wells
Light Source Xenon flash lamp

Microplate Read Time

Normal Mode

96 wells 15 seconds
384 wells 45 seconds
Dynamic Range 6 orders of magnitude
Temperature Range Ambient + 4°C up to 45°C
Temperature Unitformity (Microplate) ± 1°C @ 37°C


 Gemini™ EM Microplate Reader



Part Number

Gemini EM Microplate Reader EM
SpectraTest FL1 Fluorescence Validation Plate 0200-5060
SpectraDrop™ Micro-Volume Microplate Starter Kit 0200-6262
SpectraDrop™ Micro-Volume Microplate HTS Kit 0200-6267
Microplate Reader Shelf 9000-0756


Number of Citations*: 1,340

Latest Citations:
For a complete list, please click here.

[HTML] Paeonolum protects against MPP+-induced neurotoxicity in zebrafish and PC12 cells

XL Lu, YH Lin, Q Wu, FJ Su, CH Ye, L Shi… - BMC complementary …, 2015 -
... Quantitative measurements of fluorescence readings were determined at 485/538
nm (excitation/emission) using a SpectraMax GEMINI EM fluorescent plate reader
(Molecular Devices, USA) from cells in 96-well plates. The ...

Abnormalities in the Tricarboxylic Acid Cycle in Huntington Disease and in a Huntington Disease Mouse Model

NN Naseri, H Xu, J Bonica, JPG Vonsattel… - … of Neuropathology & …, 2015 -
Close Window. Close Window. Enter your Email address: Wolters Kluwer Health may email
you for journal alerts and information, but is committed to maintaining your privacy and will
not share your personal information without your express consent. ...

Oxidative stress involving changes in Nrf2 and ER stress in early stages of Alzheimer's disease

SI Mota, RO Costa, IL Ferreira, I Santana… - … et Biophysica Acta (BBA …, 2015 - Elsevier
Oxidative stress and endoplasmic reticulum (ER) stress have been associated with Alzheimer's
disease (AD) progression. In this study we analyzed whether oxidati.

The Mechanism of Melanocytes-Specific Cytotoxicity Induced by Phenol Compounds Having a Prooxidant Effect, relating to the Appearance of Leukoderma

T Nagata, S Ito, K Itoga, H Kanazawa… - BioMed research …, 2015 -
... The fluorescence intensity of the medium containing the solubilized cells was measured at an
exciting wavelength of 485 nm and an emission wavelength of 530 nm with a fluorescence
microplate reader (Gemini EM fluorescence microplate reader) (Molecular Devices). ...

A Novel, Single Step, Highly Sensitive In-Vitro Cell-Based Metabolic Assay Using Honeycomb Microporous Polymer Membranes

S Velayudhan, PR Kumar… - Journal of Biomedical …, 2015 -
... The release of FDA and PI embedded in the membrane was periodically monitored by carefully
pipetting 100 l of PBS into a 96-well black plate and subsequently quantifying the fluorescence
on a spectroflu- orimeter (SpectraMax Gemini EMMolecular Devices) equipped with ...

l-Histidine-based organoclays for the storage and release of therapeutic nitric oxide

AC Fernandes, ML PintoF Antunes… - Journal of Materials …, 2015 -
... Then, 10 μL alamarBlue® was added directly to each well, the plates were incubated
at 37 °C for 4 h, and the fluorescence signal was then measured on a Spectra Max
Gemini EM from Molecular Devices with SoftMax Pro software. ...

The Janus face of α-toxin: a potent mediator of cytoprotection in staphylococci-infected macrophages

J Koziel, D Chmiest, D Bryzek, K Kmiecik… - Journal of innate …, 2015 -
... aliquots of supernatant into buffer (40 mM Pipes, 20% sucrose, 200 mM NaCl, 0.2% CHAPS,
2 mM EDTA) containing DEVD-AFC and recording increased fluorescence (λ ex = 350 nm, λ
em = 460 nm) for released AFC using a Spectra Max Gemini EM (Molecular Devices). ...

Thiol-mediated multiple mechanisms centered on selenodiglutathione determine selenium cytotoxicity against MCF-7 cancer cells

T Tobe, K Ueda, M Ando, Y Okamoto… - JBIC Journal of Biological …, 2015 - Springer
... Fluorescence of resazurin was recorded at an excitation wavelength of 544 nm and emission
wavelength of 612 nm using a plate reader (Gemini EMMolecular Devices, Sunnyvale, CA, USA).
Data were obtained from three independent experiments. Measurement of apoptosis ...

Selenoglycoproteins attenuate adhesion of tumor cells to the brain microvascular endothelium via a process involving NF-κB activation

JK Wrobel, JJ Choi, R Xiao, SY Eum… - The Journal of nutritional …, 2015 - Elsevier
... The adherence of calcein-labeled cancer cells was quantified by fluorescence measurements
(Gemini EM Fluorescence Microplate Reader; Molecular Devices, Sunnyvale, CA, USA) of
endothelial monolayers using an excitation of 485 nm and an emission of 530 nm. ...

Interference of doxycycline pretreatment in a model of abdominal aortic aneurysms

KM Mata, C Tefé-Silva, EM Floriano… - Cardiovascular …, 2015 - Elsevier
... CaCl 2 ). This activity was evaluated using a microplate fluorimeter (at λ excitation of 495nm,
λ emission of 515nm; Gemini EMMolecular Devices, Sunnyvale, CA, USA) after 120 min of
incubation at 37°C because the maximal dequenching of the substrate occurred at this time. ...


* as of June 26, 2015. Source: Google Scholar.