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Comparison of Microplate Readers with Fluorescence Detection

Features
Fluorescence
Absorbance
Luminescence
Time-resolved fluorescence (TRF)
HTRF Certified
Fluorescence Polarization
Wavelength range
Bandwidth
Tunable wavelength selection/ monochromator
Endpoint
Kinetic
Spectral scanning
Well scanning
Reading speed (sec)
Microplate formats
Integrated multi-channel pipettor
Temperature control
Top reading
Bottom reading
Cuvette port
Validation tools
Robotics / automation
Fluorescence
Absorbance
Luminescence
Time-resolved fluorescence (TRF)
HTRF Certified
Fluorescence Polarization
Wavelength range
FL 250-850 nm, Abs 30 - 1000 nm, Lum 300-850 nm
Bandwidth
Adjustable: 9/15 nm (ex), 15/25 nm (em)
Tunable wavelength selection/ monochromator
Endpoint
Kinetic
Spectral scanning
Well scanning
Reading speed (sec)
Microplate formats
6 to 1536 wells°
Integrated multi-channel pipettor
Temperature control
Top reading
Bottom reading
Cuvette port
Validation tools
Robotics / automation
Fluorescence
Absorbance
Luminescence
+
Time-resolved fluorescence (TRF)
+
HTRF Certified
+
Fluorescence Polarization
+
Wavelength range
FL 250 - 850, Abs 200 - 1000
Bandwidth
9/9 or 9/15+
Tunable wavelength selection/ monochromator
Endpoint
Kinetic
Spectral scanning
Well scanning
Reading speed (sec)
15-17+
Microplate formats
6-384
Integrated multi-channel pipettor
Temperature control
Top reading
Bottom reading
+
Cuvette port
Validation tools
Robotics / automation
Fluorescence
Absorbance
Luminescence
Time-resolved fluorescence (TRF)
*
HTRF Certified
Fluorescence Polarization
Wavelength range
FL 250 - 850 ex, 250-850 em
Bandwidth
9/9
Tunable wavelength selection/ monochromator
Endpoint
Kinetic
Spectral scanning
Well scanning
Reading speed (sec)
15
Microplate formats
6-384
Integrated multi-channel pipettor
Temperature control
Top reading
Bottom reading
Cuvette port
Validation tools
Robotics / automation
Fluorescence
Absorbance
Luminescence
Time-resolved fluorescence (TRF)
*
HTRF Certified
Fluorescence Polarization
Wavelength range
FL 250 - 850 ex, 360 - 850 em
Bandwidth
9/9
Tunable wavelength selection/ monochromator
Endpoint
Kinetic
Spectral scanning
Well scanning
Reading speed (sec)
15
Microplate formats
6-384
Integrated multi-channel pipettor
Temperature control
Top reading
Bottom reading
Cuvette port
Validation tools
Robotics / automation
Fluorescence
Absorbance
Luminescence
Time-resolved fluorescence (TRF)
HTRF Certified
Fluorescence Polarization
Wavelength range
FL 250 - 850 ex, 250 - 850 em; Abs 200 - 1000
Bandwidth
9/15
Tunable wavelength selection/ monochromator
Endpoint
Kinetic
Spectral scanning
Well scanning
Reading speed (sec)
17
Microplate formats
6-384**
Integrated multi-channel pipettor
***
Temperature control
Top reading
Bottom reading
Cuvette port
Validation tools
Robotics / automation
Fluorescence
Absorbance
Luminescence
Time-resolved fluorescence (TRF)
+
HTRF Certified
Fluorescence Polarization
+
Wavelength range
FL 230 - 750 nm + Abs 230 - 650 nm +
Bandwidth
Tunable wavelength selection/ monochromator
Endpoint
Kinetic
Spectral scanning
Well scanning
Reading speed (sec)
18 seconds/96 well 37 seconds/384 well
Microplate formats
FilterMax F3: 96 - 384 well FilterMax F5: 6 - 1536 well
Integrated multi-channel pipettor
Temperature control
+
Top reading
+
Bottom reading
Cuvette port
Validation tools
Robotics / automation

 

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* Secondary modes

** 96- and 384-well with pipetting

*** 8- and 16-channel pipettor

+ Varies by model

° 1536 detection available via detection cartridges.