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CloneSelect Imager

Verify monoclonality and monitor cell growth over time with objective,
label-free imaging

Rapid confirmation of monoclonality and subsequent monitoring of cell growth are important for a number of applications including antibody discovery and cell line development. Conventional techniques are problematic because they are both time consuming and subjective. The CloneSelect™ Imager, a high-throughput automated imaging system, was specifically designed to address these workflow needs through rapid and objective acquisition and analysis of images.

  • Acquire and analyze plates for cell confluency in <90 sec
  • Compatible with 6- to 384-well plates
  • Successfully used on suspension and adherent cell lines such as CHO, HEK, hybridomas, Per.C6®, Jurkat, SupT1, H-4-II-E, MCF-7, JT29, DLD1, and KB31

                        

A Streamlined Process: Image, Analyze, Report

1. IMAGE
  • Image in label-free manner using white light and/or with fluorescence for additional applications
  • Scan, determine, and record cell confluence and cell number of the entire well for every well, automatically, over time
  • Screen one 96-well plate in 90 seconds
2. ANALYZe
  • Highlight cell confluency in each well at each time point automatically
  • Interactive growth curves created and displayed automatically
  • Algorithms optimized for cell detection in white light and fluorescence
3. REPORT
  • Verify monoclonality in just a few clicks with the Monoclonality Report feature
  • Track and view growth of every cell line in every well
  • Trace and store plate data electronically, and share it in PDF or Word format

 

High Resolution Enable Accurate Cell Detection 

 
  • Detect cells accurately even at challenging areas such as well edges
A single cell is shown near the well edge using the CloneSelect Imager
Single cell near well edge
A doublet of cells is captured near a microplate well edge using the CloneSelect Imager
Doublet near well edge

Fluorescence Imaging Provides Greater Certainty 

The fluorescence imaging capability adds an additional level of confidence as researchers use an optional fluorescence viability stain to track their cells.

The software can automatically perform cell count analysis on the fluorescence images,  making it easy to identify wells that contain a single cell.

Automated, rapid confluence determination of cells growing in microplates

  • Intelligent software analysis automatically calculates confluence for each imaging time point
  • Shown here is the confluence percentage of cells growing in each well of a 96-well microplate.

Confluence percentage of each well is shown in the CloneSelect Imager user interface

 

Automatically plots time course of cell growth 

  • Microplate images are acquired over time
  • Growth curve, image montage, total growth, and mean rate are generated automatically and are exportable

 

EASILY DOCUMENT MONOCLONALITY WITH A FEW CLICKS

With the ease of a few clicks, the Monoclonality Report feature organizes objective, quantitative, and qualitative data in an easily shareable report, enabling the presentation of image evidence needed to assure a high probability of clonality.

Example images from monoclonality report

 

  • Easily identify and select single-cell and artifact regions to include in report
  • Export high-resolution images of single cells, artifacts, and entire well (optional)
  • Automatically identify single cells with fluorescence version of CloneSelect Imager
  • Publish monoclonality report in PDF or Word Format

Automation Compatible

  • The CloneSelect Imager can be integrated with various 3rd party robotics to achieve high throughput 

Example: Automated delivery of colony plates from an incubator to CloneSelect Imager and back

Instrument Setup:

  1. CloneSelect Imager
  2. Robotic arm
  3. Incubator

Benefits:

  • Free up manual labor—customer can enjoy a walk-away time of 2-3 weeks
  • Reduce contamination and variation from plate handling
Click here to see more automation case studies with the CloneSelect Imager >> Click here to see more automation case studies with the CloneSelect Imager >>

 

Proof of Clonality

One of the regulatory requirements for biologics approval is to provide evidence that cell lines producing biotherapeutic proteins are derived from a single cell. The CloneSelect Imager can help in the following ways:

1. Save time and effort by reviewing wells that contain only a single colony with the intelligent "Loci of growth" function.

Instead of inspecting every single well in the plate, the “Loci of growth” function in the CloneSelect Imager software pinpoints wells that contain only 1 single colony, so users can save time and effort by only reviewing those wells.

2. Growth (image) history of each well can be tracked back to its starting point—providing evidence of monoclonality.

Monitor colony OUTgrowth

After screening and selecting colonies of interest, the CloneSelect Imager can monitor and evaluate subsequent colony outgrowth and productivity of the selected cell lines.

Track-colony-outgrowth.gif
Outgrowth of a single well after 7 days - confluence overlay viewed for each imaging timepoint

The CloneSelect Imager can also estimate the number of cells per well. Data is then exported to enable normalization of results from ELISA or cell-based assays against cell number. 

Normalize-downstream-results-to-cell-number_1.gif

Normalize-downstream-results-to-cell-number_2.gif
Correlate protein productivity and growth of cell line

 

Colony Forming Assay

wells_thb.jpg
Save time by analyzing selected wells
(highlighted in green). Red wells are excluded.

Objective, Time-Saving Analysis

  • Every well can be imaged at any time point
  • Colony number and area quantified objectively
  • Focus on wells of interest, e.g. where growth is inhibited by test compounds

Review Results and Export Data

  • Dual gating for colony area and compactness
  • View each well at different time points
  • Export colony data
review1_thb.gif
View number of colonies per well
colony_thb.jpg
Colonies are identified in well images

Assessment of Cell Viability (MTT Assay)

CloneSelect Imager offers several benefits over traditional MTT assays for assessing cell viability in response to a cytotoxic challenge.

  • Replace time-consuming conventional techniques
  • Monitor cell death over time
  • Ensure a permanent record of data
  • Avoid costly reagents - no staining required
“THIS NEW IMAGE-BASED TECHNIQUE HAS THE POTENTIAL TO REPLACE THE CUMBERSOME MTT ASSAY WHEN FAST, UNBIASED AND HIGH-THROUGHPUT CYTOTOXICITY ASSAYS ARE REQUESTED.”
ACCURATE NON-INVASIVE IMAGE-BASED CYTOTOXICITY ASSAYS FOR CULTURED CELLS MARQUES-GALLEGO ET AL., BMC BIOTECHNOLOGY 2010, 10:43
csi_tox_wells.jpg
Images of HeLa cells - wells exposed to
different level of cytotoxin
csi_tox_curve.gif
Dose response curve: decreasing cell
confluence with increasing exposure
to cytotoxin
High resolution images show growth of cells exposed to low, medium and high cytotoxin levels

Cell Migration Assay

CloneSelect Imager offers rapid, objective quantification of cell migration assays.

  • Determine migration rates, maximum migration rate and total migration area
  • Time-saving - scan a 96-well plate in <2 minutes
  • Convenient, label-free quantification
csi_migration1.gif
Export data to determine dose
response to cell mobility effectors

Review Results and Export Data

  • Obtain graphical display of migration area vs. time
  • Easy-to-read numerical and graphical output

 

 

csi_migration2.gif
Wound healing time course

 

We offer 3 different models to suit your needs and budget.

  CloneSelect Imager Standard  CloneSelect Imager HR  CloneSelect Imager FL 
Illumination
  • White light only
  • White light only
  • White light only
  • Fluorescence (up to 7 customizable fluorescence wavelengths available)
Pixel Resolution
  • 3.6 micron
  • 1.8 micron
  • 1.8 micron
Imaging Speed
  • < 90 sec for a 96-well microplate (white light only)
  • < 90 sec for a 96-well standard mode
  • < 8 minutes for a 96-well plate in high resolution mode (white light only)
  • < 10 min for a 96-well microplate (white light and fluorescence)
Autofocus Yes Yes Yes
Internal Barcode Reader Yes Yes Yes
Automation Compatible? Yes Yes Yes

*Download the specifications in the CloneSelect Imager brochure here.

*Download the specifications in the CloneSelect Imager brochure here.

CLONEselect Imager - System specifications
Imaging 
Software Dedicated imaging software pre-installed on a high-specification PC, Microsoft Windows 7
White light imaging Trans-illumination
Fluorescence imaging Optional (please contact us for details)
Data tracking 1 x internal barcode reader for data tracking of each plate
Camera High-resolution CCD camera
Imaging speed <90 sec in standard mode; <8 min in high resolution mode
Objective 4x
Resolution Standard: 3.6 micron; Maximum: 1.8 micron
Instrumentation
Source plate type Range of 6-, 12-, 24-, 96- and 384-well SBS microplates
Source plate capacity 1 x plates
Instrument dimensions 720 mm (width) x 428 mm (height) x 575 mm (depth)
Instrument weight 45 kg
regulATORY APPROVAL
Compliance CE
Quality ISO9001:2008 certified
AUTOMATION COMPATIBILITY
API suite available for robotic integration. Please contact us for details. 


 

Related Videos

Non-subjective interpretation of colony formation with the CloneSelect Imager
 

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  • Identification and Selection of GPCR Cell Lines with ClonePix 2 System

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For product configurations and pricing on CloneSelect Imager, please contact us

For instrument qualification services, please click here.