Digidata 1550B plus HumSilencer

Enhance your recording fidelity with quadruple the number of HumSilencer channels

Digidata 1550B Plus Humsilencer

Goodbye line-frequency noise. Hello biological signal.

Equipped with up to four HumSilencer™ channels, the Axon™ Digidata® 1550B Low Noise Data Acquisition System enables you to record multiple cells at once for cellular network studies without 50/60 Hz line-frequency noise.

The Digidata 1550B System has three different configurations. Please see the table below. For detailed specifications, please see the datasheet.

Part Number Description
Digidata 1550B4 Four HumSilencer channels
Digidata 1550B1 One HumSilencer channel 
Digidata 1550B0 No HumSilencer channel


Bring a new level of simplicity to your patch-clamp electrophysiology experiments!

While offering the same digitization capabilities as its predecessors, the Axon Digidata 1550B Low Noise Data Acquisition System offers up to four HumSilencer channels. This innovative feature allows scientists to record multiple cells simultaneously for higher throughput or cellular network studies without 50/60 Hz line-frequency noise. With only a single click, eliminate the noise obscuring your biological signals of interest and simplify your workflow.

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Digidata 1550B Datasheet

Digidata 1550B Application Note
Application Note


What is HumSilencer?


HumSilencer is an advanced feature that learns, adapts, and removes local 50/60 Hz line-frequency noise patterns and high-frequency harmonics from incoming signal. With a simple ON/OFF check box in Axon AxoScope or Axon Clampex software, HumSilencer enables subtraction of line-frequency noise from your signal.

Humsilencer example recordings

Learn about the HumSilencer technology >

Should a noise pattern change, such as by introduction of a new noise source, HumSilencer rapidly learns & adapts. The new noise is eliminated in less than one second and can handle amplitudes up to 20V peak-to-peak at digitizer's analog input.

HumSilencer is not a filter and has no effect on acquired signals. HumSilencer also causes no signal distortion such as frequency change, amplitude attenuation, phase shift, or DC voltage change.



Enabling noise-free cellular network studies


The Digidata 1550B comes equipped with up to four analog HumSilencer inputs, thus allowing stimulation and recording of multiple cells at once without 50/60 Hz line-frequency interference. All analog input channels can be simultaneously digitized at the highest sampling rate of 500 kHz for maximum throughput and quality. Multiple triggering options are available via hardware and software.   

With Digidata 1550B, you can now record cellular networks, without 50/60 Hz line-frequency noise, in a single digitizer while saving money, time, and hassle.  

HumSilencer elimination of 60 Hz noise on four channels
HumSilencer elimination of 60 Hz noise on four channels. Black traces: raw data; red traces: same data with HumSilencer enabled .


Basic Ion Channel Research

Basic ion channel research 
Ion channels are pore proteins that are involved in a large variety of physiological processes. Basic research in the ion channel field furthers the understanding of ion channel function as well as their roles within cellular pathways and processes. Single-channel research helps with elucidating the mechanisms underlying ion channel function on a single-molecule level. Whole-cell and perforated patch clamp recordings are typically used to investigate the action of modulators on entire populations of ion channels in the membrane of a cell. Learn more.

Cell Pathway Analysis

Cell pathway analysis
Ion channels are involved in many cell pathways and understanding the function of ion channels in response to changes in membrane potential or the presence or absence of other molecules is important in order to understand exactly how ion channels participate in normal and abnormal biological processes such as cell differentiation and migration, disease states, neuronal communications, etc. Learn more.

Disease Research - Ion channels

Disease Research
Ion channels play a role in many diseases including hypertension, cardiac arrhythmias, gastrointestinal, immune and neuromuscular disorders, pathological pain, and cancer. By understanding the exact role that ion channels play in a particular disease, researchers might be able to find a way to affect the ion channel in such a way as to alter the course of the disease.


hERG Testing - Safety Assessment

Safety Assessment
A critical part of drug discovery involves safety assessments, such as hERG testing, in order to eliminate any compounds with potential safety concerns. Conventional patch clamp has played an important role in collecting evidence for the absence of side effects before submission of a drug candidate to regulatory authorities. Even if automated electrophysiology has shifted safety testing to earlier stages in the drug discovery process, follow-up with conventional patch clamp is an essential step in the workflow. Learn more.

AxoScope 10.6 Software

AxoScope 10.6 Software is a turn-key data acquisition and analysis program for Microsoft Windows designed to replace oscilloscopes, chart recorders, and FM tape recorders. AxoScope 10.6 Software provides from eight to sixteen channels of analog acquisition and four different acquisition modes.

Data can be acquired and displayed continuously in Gap-Free mode up to the supported sampling rate of the digitizer. Other acquisition modes supported by AxoScope 10.6 Software are threshold-triggered Fixed-Length Events, Variable-Length Events or High-Speed Oscilloscope modes. Tags and comments can be added to the data in real time. Analog and digital output holding values can be altered.

AxoScope 10.6 Software opens Axon-format ABF data files and enables quick analysis of sections of interest with an array of browsing and basic analysis tools. Data and page layout can be reviewed before printing. Additional features include voice tags, which allow tagging of data with spoken comments*, low-pass and high-pass digital filtering of incoming data, and Store Trace, which freezes a snapshot of a waveform on the screen for comparison with a subsequent input.

In combination with pCLAMP 10.6 Software, the Digidata® 1550B System forms the data acquisition backbone of a set-up like those used in many electrophysiology labs around the world. The Clampex Data Acquisition Module, which is part of the pCLAMP Software, makes optimal use of the features of the Digidata 1550B Low-Noise Data Acquisition System.

* Requires a sound chip in the host computer and a microphone. 

Developer Support

Software testbeds are available to help programmers write data acquisition programs for Digidata Digitizers. These testbeds include sample code and DLL drivers for Windows programs. For non-Windows programs, it is suggested that you write a C wrapper for our API.

There are four testbeds currently available through our Customer Support page:

  • The Digidata 1550B Testbed allows programs to be created to control the Digidata 1550B Digitizer. The sample application requires a Digidata 1550B Digitizer to run.
  • The Digidata 1550 Testbed allows programs to be created to control the Digidata 1550 Digitizer. The sample application requires a Digidata 1550 Digitizer to run.
  • The Digidata 1440A Testbed allows programs to be created to control the Digidata 1440A Digitizer. The sample application requires a Digidata 1440A Digitizer to run.
  • The Digidata 132x Testbed allows programs to be created to control the product line of Digidata 1320A, 1321A and 1322A digitizers. Note that the Adaptec WinASPI layer is also required.

Please note that technical support is not available for writing programs using these testbeds.


  • Up to four channels that can eliminate 50/60 Hz line-frequency noise and associated high frequency harmonics in less than one second and with a single click
  • Digitizes a wide range of input signals from -10 to +10 V
  • Eliminates a maximum noise amplitude of up to 20 V, peak-to-peak
  • 8 analog input channels to digitize acquired signals up to 500 kHz independently
  • 8 analog output channels to send command voltage output independently
  • 8 digital out channels to control the periphery equipment used in sophisticated experiments
  • Independent analog-to-digital converters for each input channel ensure low crosstalk levels and high data acquisition rates.
  • USB 2.0 connection allows operation on virtually any current PC computer running Microsoft Windows 7 or 10 (32-bit or 64-bit) Operating System, including laptops.
  • 16-bit resolution data
  • State-of-the-art signal-to-noise ratio
  • All signal connectors on the front panel for ease of access and maintenance of the electrophysiology set-up
  • Rack-mount case for tidy installation of all components in the electrophysiology set-up
  • User guide written by scientific consultants, with the assistance of Axon Instruments staff, for straightforward start-up and as an in-depth reference.

General Specifications

General Specifications


Maximum input signal 
(total of noise + signal)
±10 V
Maximum noise amplitude 20 V, peak-to-peak (on a 0 V signal)
Noise cancellation Line-frequency (50 Hz / 60 Hz) and harmonics to 10 kHz
Cancellation response time < 1 second

Analog Inputs

Number of input channels 8 single-ended
Number of ADCs 8
Sampling rates
1 Hz - 500 kHz; Maximum aggregate throughput rate is 500 kHz x 8 input channels = 4 Megasamples/s
Resolution 16-bit
Input range -10.000 V to +10.000 V
Input resistance >1 MΩ
Gain value 1

Analog Outputs

Number of channels 8
Number of DACs 8
Sampling rates 1 Hz -  500 kHz
Resolution 16-bit
Output range -10.000 V to +10.000 V
Output impedance <  0.5 Ω
Output short circuit to signal ground ±25 mA

Digital Outputs

Number of bits 8 (of 16) supported in software
SCOPE Dedicated trigger output
Output driver Advanced CMOS (AC) compatible
Output current ±4 mA source, cable
Type USB 2.0 braided
Length 3 meters

Trigger Inputs

Input type TTL compatible
TAG Rising-edge sensitive
START Rising-edge sensitive

Detail Specifications


Description Details Part Number
Axoclamp 900A Headstage HS-9A X0.1U x0.1 headstage 1-2950-0359
Axoclamp 900A Headstage HS-9A X1U x1 headstage 1-2950-0360
Axoclamp 900A Headstage HS-9A X10U x10 headstage 1-2950-0361
Axoclamp 900A Headstage VG-9A X10U x10 virtual ground headstage 1-2950-0362
Axoclamp 900A Headstage VG-9A X100U x100 virtual ground headstage 1-2950-0363
MultiClamp 700B Headstage CV-7B Patch-clamp headstage 1-CV-7B
MultiClamp 700B Headstage CV-7B/BL Bilayer headstage 1-CV-7B/BL
MultiClamp 700B Headstage CV-7B/EC Electrochemistry headstage 1-CV-7B/EC

Electrode holders, adapters, and holder components

Description Details Part Number
Electrode Holder for U-Type Headstages Fits glass pipettes with outer diameter of 1.0 - 1.7 mm 1-HL-U
Electrode Holder Replacement Caps Set of 2 polycarbonate caps for HL-U holders 1-HL-CAP
Cone Washers 1.1mm ID Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.0 - 1.1 mm 1-HLC-11
Cone Washers 1.3mm ID Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.1 - 1.3 mm 1-HLC-13
Cone Washers 1.5mm ID Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.3 - 1.5 mm 1-HLC-15
Cone Washers 1.7mm ID Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.5 - 1.7 mm 1-HLC-17
Pins 1mm for HL-U Holders Set of 3 brass pins for HL-U holders, 1mm 1-HLP-U
2mm Plugs with Solder Cups Set of 5 general purpose gold plugs, 2mm, with solder cups 1-HLP-0
Silver Wire Set of 5 Ag wires, 0.25mm diameter, 50mm long 1-HLA-005
Silicone Tubing for Silver Wire 1mm ID x 70mm Long Silicone Tubing 1-HLT-70
Silver/Silver Chloride Pellet Assemblies Set of 3 Ag/AgCl pellet assemblies 1-HLA-003
Adapter For BNC Holders To U-Type Headstages Connects BNC holders to CV and HS headstages with threaded collets (U-type) 1-HLB-U
Right-Angle Adapter for HL-U Electrode Holders Fits CV and HS headstages with threaded collets (U-type) 1-HLR-U

Model Cells

Description Details Part Number
Model Cell for Oocytes Axoclamp/ GeneClamp model cell for oocytes. Connects to U-type HS series headstages 1-MCO-2U
Model Cell for TEVC / DSEVC Axoclamp/ GeneClamp model cell for two-electrode voltage clamp/ discontinuous single-electrode voltage-clamp conditions. Connects to U-type HS series headstages 1-CLAMP-1U
Model Cell for Whole Cell/ Single Channels Axopatch/ GeneClamp/ MultiClamp model cell for whole-cell / single-channel patch-clamp conditions. Connects to U-type CV series headstages 1-PATCH-1U
Model Cell for Bilayers Axopatch/ GeneClamp/ MultiClamp model cell for bilayer conditions. Connects to U-type CV series headstages 1-MCB-1U


Description Details Part Number
Cable To Connect Axoclamp 2 Headstages To Axoclamp 900A Amplifier Allows Axoclamp 2 headstages (HS-2, VG-2) to be used on Axoclamp 900A Amplifiers 1-2100-0934


Description Details Part Number
SoftPanel Amplifier Control Unit Provides physical knob and button control for computer-controlled Axoclamp 900-series and MultiClamp 700-series amplifiers. Requires a USB connection. 1-SOFTPANEL (USB)
Remote Buzz For Axoclamp 900A Hand-held buzz duration control for Axoclamp 900A Amplifier (1-50ms) 1-2950-0366
Silver/Silver Chloride Pellet Assemblies Set of 3 Ag/AgCl pellet assembly and Ag wire 1-HLA-003

Number of Citations*: 19,000

Latest Citations:

For a complete list of citations, please click here.

An optogenetics-and imaging-assisted simultaneous multiple patch-clamp recording system for decoding complex neural circuits

G Wang, DR Wyskiel, W YangY Wang, LC Milbern… - Nature protocols, 2015 -
... The most recent versions of DAQ boards from Molecular Devices are the Axon
Digidata 1440 board, which has four D/A channels and 16 A/D channels, and the
Axon Digidata 1550 board, which has eight D/A channels and ei.

Dorsal root ganglion myeloid zinc finger protein 1 contributes to neuropathic pain after peripheral nerve trauma

Z Li, X Gu, L SunS Wu, L Liang, J Cao, BM Lutz… - Pain, 2015 -
Advertisement. Close Window. Close Window. Enter your Email address: Wolters Kluwer Health
may email you for journal alerts and information, but is committed to maintaining your privacy
and will not share your personal information without your express consent. ...

Osmotic Edema Rapidly Increases Neuronal Excitability Through Activation of NMDA Receptor-Dependent Slow Inward Currents in Juvenile and Adult Hippocampus

K Lauderdale, T Murphy, T Tung, D Davila… - ASN neuro, 2015 -
... Electrophysiology. Whole-cell patch clamp recordings were made using a Multiclamp 700B
amplifier and Multiclamp Commander software, with either a DigiData 1550 or DigiData 1440A
digitizer, and Clampex 10.2–10.4 software (Molecular Devices, Sunnyvale, CA). ...

Dorsal root ganglion myeloid zinc finger protein 1 contributes to neuropathic

Z Li, X Gu, L SunS Wu, L Liang, J Cao, B Marie - 2015 -
... 320 mOsm). Signals were filtered at 1 kHz and digitized by using a DigiData 1550 with pClamp
10.4 software (Molecular Devices). ... An online P/4 leak subtraction was performed to eliminate
leak current contribution. The data were stored on computer by a DigiData 1550 ...

Nitric Oxide Signaling Is Recruited As a Compensatory Mechanism for Sustaining Synaptic Plasticity in Alzheimer's Disease Mice

S Chakroborty, J Kim, C Schneider… - The Journal of …, 2015 - Soc Neuroscience
... brain slices (400 μm) were placed in an interface chamber (Harvard Apparatus) and perfused
at 1.5 ml/min with aCSF equilibrated with 95% O 2 /5% CO 2 at room temperature (27°C). Data
were acquired at 10 kHz using pClamp 9.2 software with a DigiData 1322 AD converter ...

Cell Type-Specific Separation of Subicular Principal Neurons during Network Activities

J Eller, S Zarnadze, P Bäuerle, T Dugladze, T Gloveli - 2015 -
... The LFP recordings in the intact and isolated subiculum were amplified, digitized with a sampling
rate of 10 kHz (Digidata 1322A, Axon Instruments) and analyzed with the pClamp software
package (notch filter 50 Hz, butterworth filter 2–2000 Hz; Axon Instruments). ...

Actions of Bupivacaine, a Widely Used Local Anesthetic, on NMDA Receptor Responses

MA Paganelli, GK Popescu - The Journal of Neuroscience, 2015 - Soc Neuroscience
... intracellular Mg 2+ block. Currents were amplified and filtered at 2 kHz (Axopatch
200B), sampled at 5 kHz (Digidata 1440A) and stored as digital files using
pClamp10.2 software (Molecular Devices). Macroscopic current peak ...

Excitation of Tuberoinfundibular Dopamine Neurons by Oxytocin: Crosstalk in the Control of Lactation

V Briffaud, P Williams, J Courty… - The Journal of …, 2015 - Soc Neuroscience
... The signal was amplified and low-pass filtered on-line at 10 kHz, digitized (Digidata
1322A or Digidata 1440A, Molecular Devices), and stored on a personal computer
using Clampex v9.2 or 10.3 software (Molecular Devices). ...

Loss of F-box Only Protein 2 (Fbxo2) Disrupts Levels and Localization of Select NMDA Receptor Subunits, and Promotes Aberrant Synaptic Connectivity

G Atkin, S Moore, Y Lu, RF Nelson… - The Journal of …, 2015 - Soc Neuroscience
... 1 MΩ filled with aCSF. Recordings were digitized using an Molecular Devices 1440A
Digidata A/D converter and stored on a Dell desktop computer running pClamp 10.2
(Molecular Devices). fEPSPs were evoked by stimulating ...

Pharmacology of hSlo3 channels and their contribution in the capacitation-associated hyperpolarization of human sperm

O Sánchez-Carranza, P Torres-Rodríguez… - Biochemical and …, 2015 - Elsevier
... four-pole Bessel filter). Ion currents were digitized using a Digidata 1550 interface
(Molecular Devices, Sunnyvale, USA) and analyzed with the pCLAMP 10.5 and
SigmaPlot software suites, respectively. All experiments were ...

* as of November 13, 2015. Source: Google Scholar.  Search results include "Digidata" and "Digidata 1550".  

Specifications for the Digidata 1550B are based upon previous Digidata series (1550A, 1550, 1440, etc).  Applications will apply to these systems.