NEW Measure p53-MDM2 protein interaction with NanoBRET technology
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BRET (bioluminescence resonance energy transfer) is a technique for measuring protein-protein or protein-ligand interactions that involves the interaction of a bioluminescent donor and a fluorescent acceptor. NanoBRET™ technology from Promega improves upon earlier generations of BRET with increased signal, better sensitivity, and lower background, enabling detection of protein interactions within the context of living cells. Here, we describe the validation of SpectraMax® iD5 Multi-Mode Microplate Reader with NanoBRET™ PPI Control Pair, consisting of interacting protein partners p53 and MDM2.