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high-throughput ELISA workflow
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Cell Painting assay using automated workstation
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CloneSelect Imager FL with flourescent imaging

Fluorescent Method for Identifying Monoclonal Hek-293 Cells

In this application note, we present a fluorescent method for identifying monoclonal HEK-293 cells after gene editing with CRISPR-based p53 knockout plasmids that have a red fluorescent protein (RFP) marker for puromycin selection. The edited cells were seeded into 96-well plates with a single-cell printer. These were then monitored using CSI-FL to assess monoclonality and monitor growth through RFP and white light channels.

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Stem Cell Science and Regenerative Medicine
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