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Yeast Mnn9 is Both a Priming Glycosyltransferase and an Allosteric Activator of Mannan Biosynthesis

Striebeck A, et al., 3(9), 130022, Open Biol, 2013

This study reports the structure of the mannosyltransferase domain of yeast Mnn9 in complex with manganese and GDP, and shows that both the presence and the priming activity of ScMnn9 are required for the formation of the alpha-1,6-mannose backbone of mannan proteins. The interactions of ScMnn9 and ScVan1 were studied using the Octet RED384 system. Biotinylated ScMnn9 was loaded onto Streptavidin biosensors for these binding studies.

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