Therapeutic Intradermal Delivery of Tumor Necrosis Factor-Alpha Antibodies Using Tip-Loaded Dissolvable Microneedle Arrays

Korkmaz E, et al., pii: S1742-7061(15)00284-6, Acta Biomater, 2015

This investigation is aimed at finding a biologically effective means of delivering anti-TNF- α antibodies to the intradermal microenvironment of the skin in humans. These antibodies are potent tumor necrosis factor (TNF) inhibitors and efficient therapeutics for many inflammatory diseases. Due to inefficiencies pertaining to existing methods, the authors evaluated the applicability of tip-loaded dissolvable microneedle arrays (MNAs) for localized intradermal delivery of the antibodies. MNAs with microneedles that incorporate antibodies in the needle tip were generated from carboxymethylcellulose (CMC). An Octet QK system (Pall ForteBio) was used to verify the activity of anti-TNF- α Ab in tip-loaded CMC-MNAs. A biotinylated version of the recombinant mouse TNF-α protein immobilized onto Streptavidin biosensors was used against a dilution series of dissolved MNAs.

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