The Yeast Ess1 Prolyl Isomerase Controls Swi6 and Whi5 Nuclear Localization

Atencio D, et al., pii: g3.113.008763v1, G3 (Bethesda), 2014

This study outlines the genetic screening and functional binding analysis of the Ess1 prolyl isomerase in Saccharomeyces cerevisiae to better understand the mechanism of how this protein influences RNA polymerase II activity, transcriptional regulation and chromatin modification. Kinetic binding studies were performed on the Octet RED system using Streptavidin biosensors to analyze Ess1 binding and isomerization of phosphor-Ser-Pro motifs resulting in conformational changes that impact nuclear import and export of cell-cycle control genes.

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