The Novel Human Beta-defensin 114 Regulates Lipopolysaccharide(LPS)-mediated Inflammation and Protects Sperm from Motility Loss

Yu, H, et al., 288(17), 12270-82, J Biol Chem, 2013

The authors characterize the recombinant human beta-defensin DEFB114 peptide, demonstrating its anti-microbial potential, lipopolysaccharide (LPS) binding and neutralization, and anti-inflammatory effects in vitro and in vivo. Data suggest that DEFB114 could also protect human sperm from motility loss when challenged with LPS. Affinity measurements of interaction between LPS and DEFB114 were performed using the Octet system. Purified DEFB114 was biotinylated and loaded onto Streptavidin biosensors, followed by association/dissociation with purified LPS.

Read More