Switchable Elastin-like Polypeptides that Respond to Chemical Inducers of Dimerization

Dhandhukia, J, et. al., 14(4), 976-85 , Biomacromolecules, 2013

A strategy for designing elastin-like polypeptides (ELPs) that respond to specific chemical substrates is presented. The authors engineered a construct (FKBP-ELP) consisting of an engineered protein polymer linked to a fusion protein that homodimerized upon binding of a bifunctional chemical inducer of dimerization (CID). Association/dissociation kinetics and the affinity constant for CID binding to FKBP-ELP were characterized on the BLItz system using biotinylated FKBP-ELP loaded onto Streptavidin biosensors.

Read More