Structure of the MST4 in Complex with MO25 Provides Insights into its Activation Mechanism

Shi, Z, et al., 21(3), 449-61, Structure, 2013

This study focusses on the activation of STE20-like kinase MST4 by the scaffold protein MO25. The Octet RED96 system was used to detect direct interactions between recombinant MO25 and various MST4 fragments. The MST4 fragments were biotinylated and loaded onto Streptavidin biosensors. Association and dissociation rate constants were determined. The Octet RED96 system next was used for mutational analysis of the MST4-MO25 interface using a similar experimental approach.

Read More