Structure of Bradavidin - C-Terminal Residues Act as Intrinsic Ligands

Leppiniemi, J., et al., 7(5), e35962, PLoS ONE, 2012

The biotin-binding pocket occupying peptide (Brad-tag) serves as an intrinsic stabilizing ligand in wild-type bradavidin. The binding of Brad-tag to core-bradavidin was analysed by isothermal titration calorimetry and on the Octet RED 384 system. Anti-Penta HIS biosensors were used to immobilize His-tagged Brad-tag for these studies.

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