Structure of a Classical Broadly Neutralizing Stem Antibody in Complex with a Pandemic H2 Influenza Virus Hemagglutinin

Dreyfus C, Ekiert D C, Wilson I A, 87(12), 7149-54 , J Virol, 2013

The authors present characterization studies on C179, the first antibody identified to cross-neutralize multiple subtypes of influenza A viruses. For kinetic analysis, the Octet RED system was used. Biotinylated hemagglutinins were loaded onto Streptavidin biosensors and incubated with varying concentrations of C179 Fab. To explore the breadth of C179, the authors evaluated C179 Fab binding to a large panel of hemagglutinins, including subtypes H1-H17 of influenza A.

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