Structural Mechanism of ER Retrieval of MHC Class I by Cowpox

McCoy, W. H., et al., 10(11):e1001432, PLoS Biol, 2012

The authors performed structural and functional studies to elucidate the mechanism by which the cowpox virus-encoded protein CPXV203 blocks normal trafficking of MHCI from the endoplasmic reticulum to the plasma membrane in order to evade the immune response. Binding studies with the Octet and Biacore systems were run to determine binding affinities of CPXV203 to different MHCI molecules in a variety of buffer conditions to mimic the ER/golgi environments. Streptavidin biosensors were used.

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