Structural Insights into the Regulation of Cohesion Establishment by Wpl1

Chatterjee, A, et al., 32(5), 677-87, EMBO J , 2013

This paper presents structural and biochemical studies of the Wpl1 protein, a member of the cohesin complex. Binding affinities for Wpl1259-647 (corresponding to residues 271-635 of budding yeast Wpl1) and ScSm3-AH (variant of the cohesin protein Smc3) were determined using the Octet RED system. The Wpl1259-647 was amine-coupled to the biosensor surface for affinity measurements.

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