Structural determinants for binding of Sorting Nexin 17 (SNX17) to the cytoplasmic adaptor protein Krev Interaction Trapped 1 (KRIT1)*

Stiegler AL, et al., pii: jbc.M114.584011, J Biol Chem, 2014

The authors performed biochemical, crystallographic and biophysical analysis to better characterize the interaction between the cytoplasmic adaptor proteins nexin 17 and KRIT1 and understand their roles in the endosomal sorting process. Mutagenesis and biochemical analysis were performed to pinpoint specific regions and motifs that are key sites for promoting interaction. Binding studies were performed on the Blitz system and Anti-GST biosensors were used.

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