Structural Bases for the Interaction of Frataxin with the Central Components of Iron-Sulphur Cluster Assembly

Prischi, F., et al., 1, 95, Nature Communications, 2010

Frataxin is an essential iron-binding protein that is highly conserved in most organisms, from bacteria to humans. Reduced expression levels of this protein are sufficient to induce Friedreich's ataxia, a relentless and currently incurable neurodegenerative disease. The authors performed experiments using various biophysical techniques such as small-angle X-ray scattering (SAXS), nuclear magnetic resonance (NMR), isothermal titration calorimetry (ITC) and BLI to dissect the intractions of frataxin with important proteins in iron-related signaling pathways. Octet RED platform assays with Streptavidin biosensors helped the authors decipher the binding interactions and clarify the binding mechanism in play. They also found excellent correlation between Octet instrument data and ITC data.

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