Structural and Pharmacological Characterisation of Novel Potent and Selective Monoclonal Antibody Antagonists of Glucose-Dependent Insulinotropic Polypeptide Receptor

Ravin P, et al., 288, 19760-19772, J Biol Chem, 2013

The authors describe the generation and characterization of an antagonizing antibody (Gipg013) to the glucose-dependent insulinotropic polypeptide receptor (GIPr). Binding studies by BLI and SPR are included. The Octet RED system was used for ranking of clones during phage and ribosome display selection, and for the determination of Gipg013/Gipr ECD binding constants. Super Streptavidin biosensors were loaded with biotinylated GIPr ECD for the BLI studies.

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