Structural and Functional Insight into TAF1-TAF7, a Subcomplex of Transcription Factor II D

Bhattacharya S, et al., 111(25):9103-8, Proc Natl Acad Sci U S A, 2014

This article is reporting the crystal structure of the yeast TAF1/7 heterodimer and provides also a detailed biochemical analysis to probe its function. During eukaryotic transcriptional initiation, TATA binding protein-associated factors 1 and 7 (TAF1 and TAF7) together with two subunits of the transcription factor IID (TFIID) form a preinitiation complex (PIC). Current structure suggests that TAF1 is not a histone acetyltransferase (HAT). Instead, the new findings suggest new roles for the TAF1/7 complex in the regulation of PIC assembly. An Octet RED384 system equipped with SA biosensors was used to demonstrate the peptide binding to native or mutant yTAF1/7 protein complexes.

Read More