Selection and Maturation of Antibodies by Phage Display Through Fusion to pIX

M. Tornetta, R. Reddy, J.C. Wheeler, 58(1), 34-9, Methods, 2012

Functional assessment of phage-derived antibodies can be hindered by low affinities or lack of epitopic diversity. The authors describe an approach to managing primary hits from pIX-mediated phage libraries into epitope bins using the Octet system and Amine Reactive biosensors, followed by high-throughput maturation of clones to isolate epitope- and sequence-diverse panels of high-affinity binders.

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