Role of Iron and Sodium Citrate in Animal Protein-Free CHO Cell Culture Medium on Cell Growth and Monoclonal Antibody Production

Bai, Y., et al., 27(1), 209-219, Biotechnology Progress, 2011

The trend for biologics manufacturing is to reduce the use of animal-derived raw material to minimize potential risk of introducing contaminating agents in the final product. Iron plays a critical role in supporting healthy cell growth. In this work, chemically defined iron compounds were investigated for the development of animal protein-free cell culture media to support growth of CHO cells and production of monoclonal antibodies (mAb). MAb production in 96-well plates was analyzed by an Octet system using protein A biosensors.

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