Robust Neutralizing Antibodies Elicited by HIV-1 JRFL Envelope Glycoprotein Trimers in Nonhuman Primates

Chakrabarti BK, et al., 87(24), 13239-51, J Virol, 2013

This study explores DNA priming followed by a rest interval and protein boosting to present JRFL Env spikes to the immune system to better generate HIV-1 cross-clade neutralizing antibodies. Kinetic binding measurements of JRFL gp140-F trimers with selected monomeric Fabs were performed using the Octet RED system. JRFL gp140-F trimers were immobilized on Amine Reactive (AR2G) biosensors for these experiments.

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