Reverse Engineering of Vaccine Antigens Using High Throughput Sequencing-Enhanced mRNA Display

Nini Gu, et al., 2(8):857-65, Science Direct, 2015

This article reports the use of mRNA display and high throughput sequencing to identify peptides for a hepatitis C virus neutralizing monoclonal antibody (mAb41). This antibody prevents hepatitis C virus infection in cell cultures. Immunization of mice with the peptides produced mouse antibodies with neutralizing characteristics similar to the original antibody. The affinity characterizations performed on an Octet RED96 system equipped with Streptavidin biosensors allowed immobilization of biotinylated peptides. The analyte used in the binding assays consist of mAb41 antibody Fab segments prepared using a mouse IgG1 kit.

Read More