Reliable Protein Production in a Pseudomonas Fluorescens Expression System

Retallack, D., Jin, H., Chew, L., 81(2), 157-65, Protein Expression and Purification, 2012

This report discusses Pfenex's Pseudomonas fluorescens expression system. The Octet system with Protein L biosensors was used to select optimal expression strains for an antibody Fab fragment. Binding of Fab fragments to Protein L biosensors was used as an indication of proper folding. Octet system data are used to assess the impact of various host strains and expression vectors on the amount of Fab present in the soluble fraction.

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