Recognization of Receptors on Bone Marrow-derived Dendritic Cells Bound with Pholiota Nameko Polysaccharides

Li H, et al., 72:649-57, Int J Biol Macromol, 2015

Three major polysaccharides, namely PNPS-1, PNPS-2, and PNPS-3 isolated from a mushroom called Pholiota nameko were investigated for their ability to recognize receptors on bone marrow derived dendritic cells (BMDCs). The binding affinities derived from a Pall ForteBio Octet RED system suggest PNPSs ability to interact with TLR2, Dectin-1, and Mannose receptors. Biosensors with the ability to recognize Fc-tag and HIS-tag fusion proteins were used throughout the study. Some insights onto mechanism of action were also revealed based on the recognition patterns.

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