Recognition of Ubch5c and the Nucleosome by the Bmi1/Ring1b Ubiquitin Ligase Complex

Bentley, M.L., et al., 30(16), 3285-3297, The EMBO Journal, 2011

This publication studies the transcriptional repressor proteins Bmi1 and Ring1b, and characterizes their interaction with the E2 enzyme UbcH5c and the nucleosome. The Octet RED platform and Streptavidin biosensors were used. Biotinylated Bmi1/Ring1b complexes (wild type and mutants) were immobilized, and association/dissociation kinetic with UbcH5c were determined.

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