Rate, Affinity and Calcium Dependence of Nitric Oxide Synthase Isoform Binding to the Primary Physiological Regulator Calmodulin

McMurry, J.L, et al. , 278(24) 4943-4954, FEBS Journal, 2011

Calmodulin (CaM) is a calcium-binding messenger protein expressed in all eukaryotic cells. CaM is a multifunctional intermediate messenger protein that transduces calcium signals by binding calcium ions and then modifying its interactions with various target proteins. This publication describes use of an Octet QK system to probe the binding of eNOS and nNOS signal generating enzymes to CaM and their Ca<sup>2+</sup> dependance. Octet platform data are used to understand the binding equilibria driving formation of CaM-NOS complexes. Biotinylated CaM was immobilized on Streptavidin biosensors for these experiments.

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