Rapid and Sensitive Detection of Norovirus Antibodies in Human Serum with a Biolayer Interferometry Biosensor

Auer S, et al., doi:10.1016/j.snb.2015.06.088, Science Direct, 2015

This article discusses the use of Bio-Layer Interferometry (BLI) for rapid detection of antibodies in human serum that are specific to norovirus. The authors have taken measures to enhance the detection of antibodies from serum. Rapid detection using the BLI as compared to ELISA suggests robustness of the Octet platform in complex matrices such as the serum. An Octet RED384 equipped with Ni-NTA biosensors allowed capture of HIS-tagged NoV-VLPs or NoV P-particles. This article also highlights the use of diaminobenzidine (DAB) metal chelator as a method to enhance BLI signal from complex samples.

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