Protection of Calves by a Prefusion-stabilized Bovine RSV F Vaccine

Zhang B, et al., doi:10.1038/s41541-017-0005-9, NPJ Vaccines, 2017

The Bovine respiratory syncytial virus (bRSV) is the leading cause of respiratory disease in calves. bRSV is closely related to human RSV (hRSV), which cause severe respiratory illness in young children. Authors have recently reported a structure-based design to engineer thermostable versions of the pre-fusion (pre-F) hRSV fusion (F) glycoprotein. Reported in this article is the use of structure-based design, antigenic characterization, and X-ray crystallography to develop a prefusion-stabilized bRSV F vaccine. Pre-F-stabilizing mutations previously designed for hRSV F were transplanted into bRSV F. The binding kinetics of each purified bRSV F glycoprotein immunogen against D25, AM14, MPE8, and Mz antibodies were studied by using Bio-Layer Interferometry (BLI). A Pall ForteBio Octet RED384 instrument equipped with Ni-NTA Biosensor probes was used to perform all BLI assays. Ni-NTA sensor tips were immobilized with bRSV F variants and incubated in various Fabs in solution. KD values were determined using a 1:1 binding model. Overall results of this investigation demonstrate that pre-F-stabilized DS2 bRSV F induces high levels of neutralizing antibodies in calves. Furthermore, authors suggest that protection against hRSV may be boosted significantly in humans by vaccination through DS2-like hRSV F immunogens.

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