Post-Translational Modifications and Lipid Binding Profile of Insect Cell-Expressed Full-Length Mammalian Syt1

Vrljic, M., et al., 50 (46), 9998-10012, Biochemistry, 2011

This publication descibes the expression, purification, and biochemical characterization of Synaptotagmin 1 (Syt1), a protein involved in SNARE-mediated synptic vesicle fusion in neurons. The binding of various liposomes to Syt was assessed using biotinylated Syt1 calcium-binding domains immobilized on Streptavidin biosensors in an Octet system. Figure 5 in the publication presents association curves for liposome-biosensor binding.

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