Phosphorylation of Sli15 by Ipl1 Is Important for Proper CPC Localization and Chromosome Stability in Saccharomyces cerevisiae

Makrantoni V, et al., 9(2):e89399, PLoS One, 2014

In this study, the authors performed extensive point mutational analysis of the yeast Sli 15 protein, a key component of the chromosomal passenger complex (CPC) to better understand the mechanisms regulating eukaryotic cell division. Studies utilizing the Octet RED384 and Streptavidin biosensors to specifically examine Sli15 (wild-type compared to a series of 15 different mutant forms) binding affinity to microtubules. This work suggests that Sli 15 may play an important role with microtubules in tension sensing as opposed to chromosome biorientation.

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