Phage-derived Fully Human Antibody scFv Fragment Directed Against Human Vascular Endothelial Growth Factor Receptor 2 Blocked its Interaction with VEGF

Zhang, J., et al., 28(4), 981-9, Biotechnology Progress, 2012

VEGFR-2 is an important target for treatment of angiogenesis-related diseases. The authors discover a single-chain antibody fragment that targets extracellular domain 3 (KDR3) of human VEGFR-2. Binding of the single-chain antibody fragment to KDR3 was studied using the Octet RED system. Biotinylated KDR3 was immobilized on Streptavidin biosensors and exposed to various concentrations of the antibody fragment to determine kinetic rate constants and the dissociation constant.

Read More