pH Recycling Aqueous Two-Phase Systems Applied in Extraction of Maitake β-Glucan and Mechanism Analysis Using Low-Field Nuclear Magnetic Resonance

Hou 1, Cao X, 1405:40-8, J Chromatogr A, 2015

This article describes the utility of recyclable, pH-responsive, two-phase aqueous polymer systems (PADB and PMDM) to extract β-Glucan from G. frondosa. Low-field nuclear magnetic resonance (LF-NMR), Fourier transform infrared (FT-IR), and Pall ForteBio Octet systems were used to elucidate the partition mechanism. The Octet was used to verify the interactions between β-Glucan and different polymer materials. Due to presence of carboxyl groups, the polymers under investigation were captured via amino groups on APS sensors.

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