Peptide Microarrays Enable Rapid Mimotope Optimization for Pharmacokinetic Analysis of the Novel Therapeutic Antibody IMAB362

Schnatbaum K, et al., 9(4):545-54, Biotechnol J, 2014

IMAB362 is a therapeutic antibody used to treat gastro-esophageal and stomach cancer. The authors used a combination approach of phage display library and microarray screening to identify peptide candidates to IMAB362. Detailed kinetic binding studies of selected peptide variants were performed on the Octet RED system using Streptavidin biosensors and showed consistent and supportive results to the ELISA data to pinpoint key interactive sequences. This approach using a combination of kinetic and binding assays resulted in improved and more efficient SAR optimization of mimotopes and informative pharmacokinetic studies on IMAB362 in a mouse test system.

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