Patient-Specific Neutralizing Antibody Responses to Herpes Simplex Virus are Attributed to Epitopes on Either gD, gB, or Both and Can Be Type-Specific

Cairns T, et al., pii: JVI.01213-15, J Virol, 2015

Described herein is a detailed study involving human sera obtained from HSV (Herpes simplex virus) infected patients and tests for the ability to bind to glycoproteins and compete with anti-gD and anti-gB neutralizing mAbs. Some of the examined infected individuals had no recurrent disease. A Pall ForteBio Octet RED96 instrument equipped with biotinylated glycoproteins captured onto Streptavidin biosensors was used to investigate the serum samples. Authors describe several advantages of using the Octet system as compared to the BIAcore system that was used in their previous studies. Authors recognize Octet system as a powerful method to carry out their assays. Overall results demonstrate the significance of gD and gB serotypes as immunogens, bringing new insights for future HSV vaccine development.

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