Overexpression of Serpinb1 in Chinese Hamster Ovary Cells Increases Recombinant IgG Productivity

Lin N, et al., 193:91-9, J Biotechnol, 2015

This is a proof of concept study to generate a stable pool of overexpressing SERPINB1 using a lentiviral vector. Serpinb1 (serpin peptidase inhibitor, clade B, member 1) is an intracellular serine protease inhibitor with an ability to inhibit elastase in neutrophils while also involved in protecting bone marrow neutrophil reserves. The authors used a SERPINB1 host cell line for IgG expression for verification purposes. An increased IgG productivity and extended culture longevity were notable improvements in this study. A Pall ForteBio Octet system was used to evaluate volumetric IgG productivity.

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