Overexpression of Cryoglobulin-Like Single-Chain Antibody Induces Morular Cell Phenotype via Liquid-Liquid Phase Separation in the Secretory Pathway Organelles,2015,Upstream Processing,Quantitation of other proteins,,,FEBS J,"Hasegawa H

Hasegawa H, Patel N, Lim A, doi: 10.1111/febs.13332, FEBS J, 2015

The identification and characterization of a single-chain antibody (scFv-Fc) is reported. Upon multimerization, scFv-Fc promotes unexpected cryoglobulin-like properties. Modified scFv-Fc-stp, with a secretory tailpiece (stp) stimulates a morular cell phenotype during protein biosynthesis and also causes cryo-precipitation of secreted proteins in cell culture media causing a liquid-liquid phase separation (LLPS) event. Bio-Layer Interferometry was used to evaluate the concentration of Fc-fusion proteins in cell culture media by using a Pall ForteBio Octet RED96 instrument equipped with Protein A biosensors. Overall findings of this investigation suggest a probable reason as to why morular Russell body phenotype is seen in patients with B-lymphoproliferative disorders and plasma cell dyscrasias.

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