Origin of the Conformational Heterogeneity of Cardiolipin-bound Cytochrome C

Hong, Y., et al., 134(45), 18713-23, J Am Chem Soc, 2012

The mitochondrial phospholipid cardiolipin promotes unfolding of cytochrome C, with implications in the cellular apoptosis pathway. BLI combined with TR-FRET and FCS was utilized to understand functional partitioning between compact and extended cardiolipin-bound cytochome C conformations. Specifically, the Octet RED96 system and Streptavidin biosensors were used to determine kinetic constants of cytochrome C binding to liposomes with varied cardiolipin content.

Read More