Optimization of Cell Line Development in the GS-CHO Expression System Using a High-Throughput, Single Cell-Based Clone Selection System

Nakamura T, Omasa T, 120(3):323-9, J Biosci Bioeng , 2015

This report describes the application of high-throughput ClonePix FL system for mammalian cell line development to yield high-producing clones with high monoclonality by using CHO-K1SV cells. The CHO-K1SV is a CHO-K1 derived cell line that utilizes a glutamine synthase gene expression system (GS-CHO). An OctetQK system equipped with Protein A biosensors was used to measure antibody concentrations during this process development.

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