Optimization of Affinity, Specificity and Function of Designed Influenza Inhibitors Using Deep Sequencing

Whitehead, T.A., et al., 30(6), 543-548, Nature Biotechnology, 2012

The authors use sequence-function maps generated from deep sequencing studies to optimize the binding affinities of two computationally-designed protein inhibitors of H1N1 hemagglutinin. The Octet RED system was used to study binding specificities for the two inhibitors against a panel of 16 hemagglutinin subtypes. The inhibitors were biotinylated and loaded onto Steptavidin biosensors for these measurements.

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