Novel Mechanism of Regulation of Tomato Bushy Stunt Virus Replication by Cellular WW-Domain Proteins

Barajas D, et al., 89(4):2064-79, J Virol, 2015

Described herein is the role of cellular WW-domain proteins in regulating tomato bushy stunt virus (TBSV) replication process. The WW is a highly conserved protein domain involved in protein-protein interactions. The WW domain could also inhibit TBSV replication in yeast or in a cell-free replication assay. Kinetic experiments involve a Pall ForteBio BLItz instrument equipped with GST-tagged yeast proteins immobilized onto Anti-GST Biosensor probes. Kinetic parameters such as the association rate constant (ka), dissociation rate constant (kd), and interaction affinity constant (KD) values were determined. Overall assessment suggests a new mechanism to describe the effect of proviral factors and WW-domain proteins in the regulation of tombusviruses viral replicase complex (VRC) assembly.

Read More