Nanocell Targeting Using Engineered Bispecific Antibodies

Taylor K, et al., 7(1):53-65, MAbs, 2015

Reported in here is the development of several bispecific antibodies (BsAbs) to target the EnGeneIC delivery vehicle (EDVTMnanocell) via epidermal growth factor receptor (EGFR). Since the EDVTMnanocell is coated with lipopolysaccharide (LPS), BsAb design formats included scFvs derived from an anti-LPS antibody (1H10) and an anti-EGFR antibody ABX-EGF. Bio-Layer Interferometry was used to study the binding of mAbs and BsAbs to LPS molecules using a Pall ForteBio Octet instrument equipped with Aminopropylsilane (APS) biosensors. LPS molecules were captured on to APS Biosensor probes. Due to natural stickiness of LPS to glass and plastic, the investigators were unable to use Biacore microfluidics systems to perform this assay. Overall results provide clues for the development of new BsAbs capable of efficiently targeting nanoparticles that would play an important role in future nanomedicine.

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